Direct Renin Assay and Plasma Renin Activity Assay Compared
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چکیده
منابع مشابه
Direct renin assay and plasma renin activity assay compared.
substances produced an interference 5% compared with the serum diluted with drug cocktail diluent. The list of drugs tested appears in Supplement 1, which accompanies the online version of this Technical Brief at http:// www.clinchem.org/content/vol50/issue11/. Ion suppression was assessed as recommended by Annesley (12 ). Briefly, the response of calibrators injected into the mobile phase was ...
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Renin activity is separated from rabbit plasma by globulin precipitation, dialysis, and DEAE cellulose column chromatography with a Tris chloride buffer system and acid elution, followed by ultrafiltration. The plasma renin extract contains no renin substrate and no angiotensinase activity can be detected after incubation with angiotensin for 200 hr. Incubation with a known concentration of rab...
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Featured Article: Fyhrquist F, Soveri P, Puutula L, Stenman U-H. Radioimmunoassay of plasma renin activity. Clin Chem 1976;22:250 – 6. In the early 1970s there was a growing need for reliable and clinically useful methods for the determination of the activity of the renin-angiotensin system. Bioassay methods for renin activity, although valuable in early research, were too cumbersome and unreli...
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BACKGROUND Our renin IRMA overestimated renin in plasmas with high prorenin-to-renin ratios. We suspected that the overestimation of renin was caused less by cross-reactivity of the renin-specific antibody with prorenin than by a conformational change of prorenin into an enzymatically active form during the assay. METHODS Because the inactive form of prorenin converts slowly into an active fo...
متن کاملBritain A Radiochemical Renin Assay
1. A synthetic 3-([14C]valine)-labelled tetradecapeptide renin substrate was used to measure renin concentration. Renin liberated "4C-labelled angiotensin I, which was separated from the labelled substrate by paper chromatography. The conversion ofsubstrate into angiotensin I was quantitated by liquid-scintillation counting of radioactivity. 2. The rate of conversion of the substrate into angio...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2004.033654